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Crystallization and preliminary X‐ray analysis of the tungsten‐dependent acetylene hydratase from Pelobacter acetylenicus

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Acetylene hydratase is a tungsten‐containing hydroxylase that converts acetylene to acetaldehyde in a unique reaction that requires a strong reductant. The subsequent disproportionation of acetaldehyde yields acetate and ethanol. Crystals of the tungsten/iron–sulfur protein acetylene hydratase from Pelobacter acetylenicus strain WoAcy 1 (DSM 3246) were grown by the vapour‐diffusion method in an N2/H2 atmosphere using polyethylene glycol as precipitant. Growth of crystals suitable for X‐ray analysis strictly depended on the presence of TiIII citrate or dithionite as reducing agents.
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Keywords: acetylene hydratase; oxygen‐sensitivity; tungsten hydroxylases

Document Type: Research Article

Publication date: March 1, 2005

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