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Crystallization and preliminary X‐ray analysis of reducing‐end xylose‐releasing exo‐oligoxylanase from Bacillus halodurans C‐125

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The reducing‐end xylose‐releasing exo‐oligoxylanase (Rex) from Bacillus halodurans C‐125, a novel family GH8 glycoside hydrolase, was crystallized by the hanging‐drop vapour‐diffusion method using 13.6 mg ml−1 purified Rex, 5.6%(v/v) polyethylene glycol 4000, 70 mM sodium acetate pH 4.6 and 30%(v/v) glycerol. Suitable crystals grew after incubation for 5 d at 293 K. The crystals belonged to space group P212121, with unit‐cell parameters a = 52.69, b = 86.02, c = 87.92 Å. X‐ray diffraction data were collected at a resolution of 1.35 Å.
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Keywords: glycoside hydrolases; reducing‐end xylose‐releasing exo‐oligoxylanase

Document Type: Research Article

Publication date: March 1, 2005

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