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Crystallization of a truncated soluble human semicarbazide‐sensitive amine oxidase

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Human semicarbazide‐sensitive amine oxidase (SSAO) is a homodimeric copper‐containing monoamine oxidase that occurs in both a membrane‐bound and a soluble form. SSAO is also known as vascular adhesion protein‐1 (VAP‐1). A truncated soluble form of human SSAO (comprising residues 29–763) was expressed in human embryonic kidney 293 cells and purified to homogeneity. Tetragonal crystals were obtained and a data set extending to 2.5 Å was collected. The crystals are merohedrally twinned and the estimation of the twinning fraction was complicated by pseudo‐symmetry and the anisotropic character of the crystals. Using a recently developed method for twinning detection that is insensitive to phenomena such as anisotropy or pseudo‐symmetry [Padilla & Yeates (2003), Acta Cryst. D59, 1124–1130], the twinning fraction was estimated to be 0.3. The structure was eventually solved by molecular replacement in space group P43.
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Keywords: crystal twinning; semicarbazide‐sensitive amine oxidase; vascular adhesion protein‐1

Document Type: Research Article

Publication date: March 1, 2005

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