Skip to main content
padlock icon - secure page this page is secure

Calponins Are Recruited to Actin‐Rich Structures Generated by Pathogenic Escherichia coli, Listeria, and Salmonella

Buy Article:

$52.00 + tax (Refund Policy)

The ingestion of enteropathogenic Escherichia coli (EPEC), Listeria monocytogenes, or Salmonella enterica serovar Typhimurium leads to their colonization of the intestinal lumen, which ultimately causes an array of ailments ranging from diarrhea to bacteremia. Once in the intestines, these microbes generate various actin‐rich structures to attach, invade, or move within the host intestinal epithelial cells. Although an assortment of actin‐associated proteins has been identified to varying degrees at these structures, the localization of many actin stabilizing proteins have yet to be analyzed. Here, we examined the recruitment of the actin‐associated proteins, calponin 1 and 2 at EPEC pedestals, L. monocytogenes actin clouds, comet tails and listeriopods, and S. Typhimurium membrane ruffles. In other systems, calponins are known to bind to and stabilize actin filaments. In EPEC pedestals, calponin 1 was recruited uniformly throughout the structures while calponin 2 was enriched at the apical tip. During L. monocytogenes infections, calponin 1 was found through all the actin‐rich structures generated by the bacteria, while calponin 2 was only present within actin‐rich structures formed by L. monocytogenes near the host cell membrane. Finally, both calponins were found within S. Typhimurium‐generated membrane ruffles. Taken together, we have shown that although calponin 1 is recruited to actin‐rich structures formed by the three bacteria, calponin 2 is specifically recruited to only membrane‐bound actin‐rich structures formed by the bacteria. Thus, our findings suggest that calponin 2 is a novel marker for membrane‐bound actin structures formed by pathogenic bacteria. Anat Rec, 301:2103–2111, 2018. © 2018 Wiley Periodicals, Inc.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Listeria monocytogenes; Salmonella enterica serovar Typhimurium; actin; calponin; enteropathogenic Escherichia coli

Document Type: Research Article

Publication date: December 1, 2018

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more