Skip to main content
padlock icon - secure page this page is secure

Canine Oral Mucosal Fibroblasts Differentiate into Osteoblastic Cells in Response to BMP‐2

Buy Article:

$47.00 + tax (Refund Policy)

Abstract

Several lines of evidence show that transplantation of osteoblastic cells or genetically engineered nonosteogenic cells expressing osteoblast‐related genes into bone defects effectively promotes bone regeneration. To extend this possibility, we investigated whether oral mucosal fibroblasts are capable of differentiating into osteoblastic cells by conducting in vitro and in vivo experiments. We investigated the effects of bone morphogenetic protein‐2 (BMP‐2) on osteoblast differentiation of cultured fibroblasts isolated from canine buccal mucosa. We also transplanted green fluorescence protein (GFP)‐expressing fibroblasts with gelatin/BMP‐2 complexes into the subfascial regions of athymic mice, and investigated the localization of GFP‐positive cells in the ectopically formed bones. The cultured canine buccal mucosal fibroblasts differentiated into osteoblastic cells by increasing their alkaline phosphatase (ALP) activity and Osteocalcin, Runx2, and Osterix mRNA expression levels in response to BMP‐2. Transplantation experiments of GFP‐expressing oral mucosal fibroblasts with gelatin/BMP‐2 complexes revealed that 17.1% of the GFP‐positive fibroblasts differentiated into ALP‐positive cells, and these cells accounted for 6.2% of total ALP‐positive cells in the ectopically formed bone. This study suggests that oral mucosal fibroblasts can differentiate into osteogenic cells in response to BMP‐2. Thus, these cells are potential candidates for cell‐mediated bone regeneration therapy in dentistry. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Research Article

Affiliations: 1: Section of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan 2: Section of Removable Partial Denture Prosthodontics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan

Publication date: 01 August 2012

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more