Skip to main content
padlock icon - secure page this page is secure

Organ Perfusion and Mass Spectrometry: A Timely Merger for Drug Development

Buy Article:

$63.00 + tax (Refund Policy)

Organ perfusion techniques bridge the methodological gap between in vivo studies on the one hand and in vitro studies on the other. In drug candidate selection and subsequent development the differences between these systems should be considered carefully in study design, as one approach may be more suitable than the other depending on the question(s) being asked and, in particular, how the data will be used.

This article is not concerned with the mechanics, the surgery or composition of perfusates as there are numerous reviews / books covering these aspects. Instead, using perfused gut, liver, lung, kidney and brain as examples, the emphasis is on the usefulness (or otherwise) of the data generated with respect to drug absorption, metabolism, pharmacokinetics (PK) and the factors which affect these parameters.

Perfusion systems are not difficult to set up but do require ‘high maintenance’ for routine use. For this reason they have been used sparingly by the pharmaceutical industry mainly for problem solving or mechanistic studies. The latter part of this article shows how simultaneous dosing of numerous compounds followed by multiple - component analysis using LC / MS / MS has proved to be an effective way to improve the throughput of absorption, pharmacokinetics and metabolism screening ex vivo.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Mass Spectrometry; pharmacokinetics (PK)

Document Type: Review Article

Publication date: 01 January 2002

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more