Skip to main content
padlock icon - secure page this page is secure

High Resolution Tandem Mass Spectrometry for Structural Biochemistry

Buy Article:

$63.00 + tax (Refund Policy)

Tandem mass spectrometry (MS / MS) is a well-established technique for determining biomolecular primary sequences. The main advantages of MS / MS analysis compared to more traditional sequencing techniques are speed of analysis, sensitivity, high resolution and high mass accuracy. Currently, the highest performance (highest resolution, highest mass accuracy) mass analyzer is the Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. The FT-ICR mass analyzer offers several alternative techniques for tandem mass spectrometry: for example “heating” techniques, such as sustained off-resonance irradiation collision-induced dissociation (SORI-CID), infrared multiphoton dissociation (IRMPD), blackbody infrared radiative dissociation (BIRD), and the recently introduced technique electron capture dissociation (ECD).

In this review, we give an overview of FT-ICR theory, instrumentation, and performance. We also describe the different FT-ICR MS / MS techniques and discuss their capabilities and limitations for the structural biochemistry of peptides, proteins, oligonucleotides, carbohydrates, and glycoconjugates. For example, the complementarity of IRMPD and ECD for glycopeptide structural determination is demonstrated.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Tandem Mass Spectrometry; carbohydrates; cyclotron resonance (FTICR); glycoconjugates; oligonucleotides

Document Type: Review Article

Affiliations: Department of Medical Biochemistry, Goteborg University, Box 440, SE-405 30 Goteborg, Sweden

Publication date: 01 October 2003

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more