@article {Wadkins:2000:0929-8673:1,
title = "Targeting DNA Secondary Structures",
journal = "Current Medicinal Chemistry",
parent_itemid = "infobike://ben/cmc",
publishercode ="ben",
year = "2000",
volume = "7",
number = "1",
publication date ="2000-01-01T00:00:00",
pages = "1-15",
itemtype = "ARTICLE",
issn = "0929-8673",
url = "https://www.ingentaconnect.com/content/ben/cmc/2000/00000007/00000001/art00002",
doi = "doi:10.2174/0929867003375461",
keyword = "actinomycin D, oxytricha nova tolemere binding protein, TFIIIA, duplex DNA dGGAATTCC2, recognition sites, Drosophila, transcription regulation, Prokaryotic, xenopus kidney cell, nuclear factor I NFI, zinc finger protein, cruciform structures, hairpin interactive agents, telomerase, cruciformn binding proteins, rRNA promoter, E coli DNA gyrase, genomic DNA, telomeres, malaria parasite P falciparum, bacteriophage N4, ssDNA, N4 virion RNA polymerase vRNAP, biological systems, single stranded DNA, DNA Secondary Structures, trancription factors, hairpin, eukartyotic species, E coli, coliphage N4 virion RNA polymerase, dsDNA, HIV1 reverse transcripase activity, HIV 1 nucleocapsid protein NC",
author = "Wadkins, R.M.",
abstract = "DNA secondary structures containing regions of single-stranded DNA have now been identified in the genomic DNA of a number of prokaryotic and eukaryotic species, including humans. Many of these secondary structures are associated with regions of DNA involved in regulation of transcription: promoters or upstream elements. The secondary structures involved appear likely to be hairpin or cruciform structures that may be recognition sites for binding of transcription factors. In the case of the coliphage N4 virion RNA polymerase, a defined hairpin in the polymerase promoter necessary for binding of the polymerase and regulation of transcription has been shown to be extruded under physiological conditions in plasmid DNA. The presence of single-stranded DNA in the promoters of several species suggests that regulatory hairpins may be involved in transcription of a number of genes. In support of this, hairpin- or cruciform-binding proteins have been identified from several species. These results imply that secondary structures in regulatory regions may be targets for drugs that bind and either block or enhance binding of proteins involved in transcription. In this review, we discuss the evidence for DNA secondary structures, particularly hairpins and cruciforms, in genomic DNA and review the studies to date of development of small molecules that can selectively bind these structures.",
}