@article {Mosafer:2018:1567-2018:1323,
title = "Cell Surface Nucleolin as a Promising Receptor for Effective AS1411 Aptamer-Mediated Targeted Drug Delivery into Cancer Cells",
journal = "Current Drug Delivery",
parent_itemid = "infobike://ben/cdd",
publishercode ="ben",
year = "2018",
volume = "15",
number = "9",
publication date ="2018-11-01T00:00:00",
pages = "1323-1329",
itemtype = "ARTICLE",
issn = "1567-2018",
url = "https://www.ingentaconnect.com/content/ben/cdd/2018/00000015/00000009/art00013",
doi = "doi:10.2174/1567201815666180724104451",
keyword = "Nucleolin, doxorubicin, targeted delivery, PLGA, AS1411 aptamer, internalization",
author = "Mosafer, Jafar and Mokhtarzadeh, Ahad",
abstract = "Background: One of the major abundant proteins in the nucleous is nucleolin that overexpressed on the cytoplasmic membrane of malignant and endothelial cells and makes it as a promising condidate for targeted drug delivery. Objectives: In this study, doxorubicin (Dox) as a chemotherapy
drug was entrapped into the Poly lacticco- glycolic acid (PLGA)-based nanoparticles (NPs). Then, the targeting ability of anti nucleolin AS1411 aptamer-targeted Dox-encapsulated PLGA-based NPs (AS1411-NPs) was investigated in high nucleolin-expressing C26 colon carcinoma and rat C6 glioma
cell lines compared with low nucleolinexpressing mouse L929 cell line. Methods: We recently first assessed the existence of cell surface nucleolin of these three different cell lines by immunocytochemistry method. We found that a large amount of nucleolin was localized in the cytoplasmic
membrane of C26 and C6 cell lines, with a very smaller amount on the surface of L929 cell line. Results: As a result, more rapidly internalization of AS1411-NPs into the C26 and C6 cells compared with L929 cells was verified. Conclusion: We think that AS1411-NPs, as a ligand, first
bind to nucleolin, as a receptor, and then the receptor-ligand complex is more efficiently incorporated into the high nucleolin-expressing cell lines through receptor-mediated endocytosis pathway.",
}