@article {Panin:2011:1872-3136:9,
title = "The Mechanism of Interacting Biologically Active Complexes Dehydroepiandrosterone- or Tetrahydrocortisol-Apolipoprotein A-I with DNA and their Role in Enhancement of Gene Expression and Protein Biosynthesis in Hepatocytes",
journal = "Current Chemical Biology",
parent_itemid = "infobike://ben/ccb",
publishercode ="ben",
year = "2011",
volume = "5",
number = "1",
publication date ="2011-01-01T00:00:00",
pages = "9-16",
itemtype = "ARTICLE",
issn = "1872-3136",
url = "https://www.ingentaconnect.com/content/ben/ccb/2011/00000005/00000001/art00002",
doi = "doi:10.2174/187231311793564315",
keyword = "chylomicrones, macrophages, BandScan program, agarose gel. UV-photo, steroid hormones, reticular, Gene Expression, adrenal cortex fascicular zone, adrenal cortex, gluconeogenesis enzymes, densitometry data, hybridization, macromolecular, UV-spectrum, bacteriophage, Endocrinology, hybridization of DNA, ultracentrifugation, 3-keto group, DNA structure, Biologically Active Complexes, sulfate, autoradiographs, blood serum, hepatocyte culture, dehydroepiandrosterone, luteinizing hormone, optical density, Radioactivity, hormones, hydroxyl group, stoichiometry, tetrahydrocortisol, ether-ethanol mixture, alcohol-ether, Protein biosynthesis, fascicular zones, electrophoresis, glucocorticoids, hepatocytes, X-ray film, eukaryotic, buffer, apolipoprotein AI, hypersensitive, leucine solution, High density lipoproteins, RNA-polymerase, gentamycin, cortisol, DNA, X-ray Scattering, ribosomal RNA",
author = "Panin, Lev and Gimautdinova, Olga and Kuznetsov, Pavel and Tuzikov, Fedor",
abstract = "Dehydroepiandrosterone (DHEA), dehydroepiandrosterone-sulfate (DHEAS) and tetrahydrocortisol (THC) with apolipoprotein A-I form the biologically active complexes able to interact specifically with eukaryotic DNA. This conjugate is highly cooperative and results in local splitting of DNA. Specific binding sites of steroid-apoA-I complexes are the (GCC/GGC)n sequences. At the sites of splitting, single-stranded regions sensitized to the action of S1-nuclease form. These regions are irregularly distributed over DNA. The formation of single-stranded DNA regions can promote the interaction with RNA-polymerase. Formation of the biologically active THC (DHEA)-apoA-I complexes is related with resident macrophages having 5- and 5 -reductase activity. These complexes greatly enhance the rate of protein biosynthesis in hepatocytes. The cortisol-apoA-I complex does not show such effect. So, the reduced forms of fascicular zone and reticular cortex adrenal zone hormones have synergism of action toward gene expression and protein biosynthesis. The intensification of tissues regeneration during the stress period as a result of given mechanism is discussed. ",
}