
Mini-Review: Recent Developments in Hydroxynitrile Lyases for Industrial Biotechnology
Hydroxynitrile lyases (HNLs) catalyze the cleavage as well as the formation of cyanohydrins. The latter reaction is valuable for the stereoselective C-C bond formation by condensation of HCN with carbonyl compounds. The resulting cyanohydrins serve as versatile building blocks for a
broad range of chemical and enzymatic follow-up reactions. A significant number of (R)- and (S)-selective HNLs are known today and the number is still increasing. HNLs not only exhibit varying substrate scope but also differ in sequence and structure. Tailor-made enzymes for large-scale manufacturing
of cyanohydrins with improved yield and enantiomeric excess are very interesting targets, which is reflected in a solid number of patents. This review will complement and extend our recent review with a strong focus on applications of HNLs for the synthesis of highly functionalized, chiral
compounds with newest literature, recent and current patent literature.
Keywords: Asymmetric synthesis; C-C coupling; HNL; biocatalysis; biotransformation; cascade reactions; chiral building blocks; cyanogenesis; cyanohydrin; enantioselectivity; enzyme engineering; hydrocyanic acid; hydroxynitrile lyase; industrial process; mandelonitrile lyase; oxynitrilase; plant enzymes; stereoselective
Document Type: Research Article
Publication date: December 1, 2013
- Recent Patents on Biotechnology publishes review articles by experts on recent patents on biotechnology. A selection of important and recent patents on biotechnology is also included in the journal. The journal is essential reading for all researchers involved in all fields of biotechnology.
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