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Engineering Enzymes for Biocatalysis

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Protein engineering techniques have been available for over two decades beginning with the development of methods for genetic engineering. Since that time, the engineering of enzymes has advanced rapidly along with a revolution in the range and efficiency of new techniques and strategies for designing and evolving proteins in the laboratory. While recent advances in high-throughput screening techniques are permitting larger libraries of enzymes to be screened more rapidly, a combination of new genetic tools and computational methods are enabling the efficient application of random mutagenesis targeted to areas of enzyme structures that are more likely to elicit the desired enhancement of their biocatalytic properties. Meanwhile the rational design of enzyme properties, in particular, by computational design is showing great potential. This review article summarises recent and important patents relating to the engineering of enzymes for biocatalysis.

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Keywords: Rational design; biocatalysis; computational design; directed evolution; enzyme engineering; protein engineering

Document Type: Research Article

Affiliations: The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.

Publication date: February 1, 2007

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  • Recent Patents on Biotechnology publishes review articles by experts on recent patents on biotechnology. A selection of important and recent patents on biotechnology is also included in the journal. The journal is essential reading for all researchers involved in all fields of biotechnology.
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