Molecularly Imprinted Polymer Sensor Based on Microporous Metal-Organic Framework for Detection of Doxorubicin Hydrochloride

Doxorubicin Hydrochloride is an antineoplastic which is widely used. At present, a sensitive electrochemical molecularly imprinted sensor was developed to detect Doxorubicin Hydrochloride based on a gold electrode modified with p-aminothiophenol (PATP) functionalized gold nanoparticles (AuNPs) in the presence of Doxorubicin Hydrochloride as template molecule. After the extraction of the template molecules, the holes which can identify and combine with Doxorubicin Hydrochloride with high efficiency are formed directly. The performance of the developed sensor was characterized by Square Wave Voltammetry using hexacyanoferrate/hexacyanoferrite ([Fe(CN)₆]^–3/–4) as redox probe. The sensor with a signal-to-noise ratio of 3 exhibits a generalized linearity between 1.72 nM to 1.72 μM and a detection limit of 0.067 nM which is relatively lower than the other methods. Compared to non-imprinted sensor without binding template molecules, the imprinted sensor reveals high selectivity and good reproducibility. Furthermore, the developed sensor was successfully applied to test the levels of Doxorubicin hydrochloride in spiked serum samples.