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Chemometric Analysis for the Determination of Methylglyoxal in Grilled Chicken Using ZnO Flakes Based Glyoxalase 1 Biosensor

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Linear sweep voltammetric behaviour of methylglyoxal was investigated using Pt/ZnO flakes/GLO 1 bio-electrode and a new analytical method was developed for the precise and accurate determination of methylglyoxal in grilled chicken sample. This method was based on the electrochemical parameters measured for the increasing concentration of methylglyoxal. The electrochemical parameters calculated from the linear sweep voltammograms served as the dependent variables. The concentrations of methylglyoxal were employed as the independent variables. For each electrochemical parameter, a non-linear calibration equation was framed and its predictive performance was evaluated. The analytical performance of the proposed models was assessed with the aid of relative prediction error, % recovery and root mean square error for cross validation. The best results were obtained with current density versus [methylglyoxal] model (RPE = 0.033, Recovery = 97.379% and RMSECV = 1.429). This calibrated non-linear model was then applied for the determination of methylglyoxal in grilled chicken samples. The recovery of methylglyoxal in spiked grilled chicken was ranging from 95.895% to 108.191%. The predicted methylglyoxal values were very similar to that of the spiked methylglyoxal values.
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Keywords: CALIBRATION EQUATION; CHEMOMETRICS; ELECTROCHEMICAL PARAMETER; LINEAR SWEEP VOLTAMMETRY; METHYLGLYOXAL; NON-LINEAR REGRESSION

Document Type: Research Article

Publication date: March 1, 2015

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