Detection of E.Coli Bacteria Using Impedance Spectroscopy and Surface Plasmon Resonance Imaging Based Biosensor
In this work, we report a biosensing approach based on immunodetection of E.coli bacteria. Two different transducers were used for quantitative analysis of antibody-bacteria interaction. Two types of antibodies were immobilized on gold surface by simple physisorption technique.
Electrochemical impedance spectroscopy was used to characterize each step of biosensor conception and SPR imaging for bacteria detection. Impedimetric and optical generated signals were tested against E.coli bacteria. The detection limit of 104 CFU/ml was reached for impedimetric
biosensor and 105 CFU/ml for SPR based biosensor.
Keywords: ANTIBODY; E.COLI; IMPEDANCE SPECTROSCOPY; SPR IMAGING
Document Type: Research Article
Publication date: 01 December 2011
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