New low-noise detection method has been developed and used for design of a new type of biosensors based on detection of nano-sized superparamagnetic particles or magnetic beads that serve as labels for biochemical reactions. The method is based on non-linear magnetization of such particles. The particles are exposed to a magnetic field having components at two frequencies f 1 and f 2. The response is measured at combinatorial frequencies f i = m · f 1 + n · f 2, where m and n are integers (one of them can be zero). The integers can be varied to get the best signal-to-noise ratio, e.g., f i = f 1 ±2 · f 2. Several readers have been designed for the particles counting and used for different immunoassay formats, including those compatible with immunoconcentration and magnetic enrichment of antigens. Registration of 0.1 ng/ml of Y. pestis antigen and 103 cell/ml of Salmonella typhimurium has been demonstrated. The developed biosensing platforms can be used for medical diagnostics, points of care, food pathogen detection, water analysis, etc.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
Document Type: Research Article
Publication date: March 1, 2007
More about this publication?
- The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Terms & Conditions
- Ingenta Connect is not responsible for the content or availability of external websites