Componential Modeling Led Construction of an Enzyme Biosensor Reinforced with Iron Oxide Nanoparticles Onto the Self-Assembled Monolayers
A componential modeling of layer by layer assembly of Cholesterol Oxidase enzyme based biosensor was carried out wherein an in silico assembled and molecular properties estimated bio-electrode with predictable characteristics was envisioned. The designed bio-electrode was matched with the experimental device prepared in laboratory. The bio-electrode thus designed and prepared was augmented with freshly prepared Iron Oxide nanoparticles providing needed stability to the real electrical device as predicted from the molecular properties and energy status in the structure-properties relationship inference. The Cysteamine self-assembled monolayers (SAM) attached to gold surface on a glass-plate were covalently bound to the enzyme, Cholesterol Monoamine Oxidase (ChOx), immobilized through Glutaraldehyde, a bi-dentate linker, to produce the sensing device on further development. The lab-prepared device was well-characterized at each stage of its preparation and the final, iron-oxide nanoparticles augmented sensing device convincingly sensed and estimated Cholesterol biomolecule's concentrations in known test samples. The developed device was found to have linearity from 50–400 mg/dL with the detection limit of 50 mg/dL. The shelf-life was about 4 weeks with over 30 estimations of test samples when device stored at 4 C. It was observed that augmentation with nanoparticles increased the shelf-life and over-all working stability of the developed biosensor which was in agreement with the predictions and was confirmed by the modeling studies. An estimation of molecular energy levels, before and after the Iron Oxide nanoparticles augmentation, showed significant changes which indicated the stabilization of the prepared final device. The pre-Iron Oxide nanoparticle assembly was at higher energy levels (–1.4 × 1030 Kcal/mol) than the nanoparticles reinforced assembly (–3.3 × 1021 Kcal/mol) contributing to the stabilized status of the final Iron Oxide nanoparticle loaded biosensing device.
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Document Type: Research Article
Publication date: February 1, 2014
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- Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
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