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9,10-(Divinyl) Anthracene Based Bright Aggregation-Induced Emission Organic Dots for HeLa Cells Imaging

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Fluorescent organic dots have been widely utilized for various biological imaging. To obtain organic dots with high quantum yield (QY), different molecules with aggregation-induced emission (AIE) property and polymers with amphiphilic are used. By encapsulating AIE molecule 9,10-divinyl anthracene (DSA) with poly(styrene-co-maleic anhydride) and poly(isobutylene-alt-maleic anhydride) (PSMA and PIMA), novel bright AIE organic dots were obtained. The prepared AIE organic dots (PSMA dots and PIMA dots) had uniform particle size distribution, spherical appearance and small particle size (about 23 nm). However, PSMA dots had higher QYs than PIMA dots, and reached about 28.9% when the mass ratio of PSMA:DSA was 1:1. After folic acid (FA) conjugating to the surface of the PSMA dots, FA-dots were obtained. FA-dots possessed uniform particle size, good optical properties, stability and biocompatibility. The FA-dots could mark the cells where folate receptors were over-expressing. In vitro cell experiment indicated that FA-dots could be endocytosed into Hela cells, and marked the cell with specific.
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Keywords: Aggregation Induced Emission; Cell Imaging; Distyrene Anthracene; Organic Dots

Document Type: Research Article

Affiliations: 1: Chongqing Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing 401331, China 2: Department of Foreign Language, Army Logistics University of PLA, Chongqing 401331, China

Publication date: April 1, 2020

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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