@article {Qing:2019:1533-4880:4109,
title = "Biosynthesis, Characterization and Biological Activities of Silver Nanoparticles from Pogostemon cablin Benth. Methanolic Leaf Extract",
journal = "Journal of Nanoscience and Nanotechnology",
parent_itemid = "infobike://asp/jnn",
publishercode ="asp",
year = "2019",
volume = "19",
number = "7",
publication date ="2019-07-01T00:00:00",
pages = "4109-4115",
itemtype = "ARTICLE",
issn = "1533-4880",
eissn = "1533-4899",
url = "https://www.ingentaconnect.com/content/asp/jnn/2019/00000019/00000007/art00054",
doi = "doi:10.1166/jnn.2019.16282",
keyword = "Anticancer Activity, Nanoparticles, Cytotoxicity, Free Radical Scavenging Activity, Biosynthesis",
author = "Qing, Sun and Shoutian, Qiu and Hongyan, Gu and Ming, Yao and Swamy, Mallappa Kumara and Sinniah, Uma Rani and Umar, Ahmad and Akhtar, Mohd Sayeed",
abstract = "This study reports the biosynthesis of silver nanoparticles (AgNPs) using methanolic leaf extract of Pogostemon cablin Benth. (Patchouli) as a reducing agent, and their potent biological (antibacterial, antioxidant and anticancer) activities. The P. cablin extract when
exposed to silver nitrate reduced silver ions to form crystalline AgNPs within 1 h of incubation at room-temperature. UV-visible spectra showed a sharp surface plasmon resonance (SPR) at around 430 nm for the biosynthesized AgNPs and the XRD pattern indicated the crystalline planes of the
face centered cubic silver. The FE-SEM analysis revealed the occurrence of predominant spherical shaped AgNPs with a huge disparity in their particle size distribution with an average size of 25 nm, while, the FTIR data confirmed the bio-reduction and capping of AgNPs by several phytocompounds
present in the methanolic leaf extract. AgNPs effectively inhibited the growth of all the tested human pathogenic bacterial strains (Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli), while, the methanolic leaf extract failed to inhibit the
growth of S. aureus and P. aeruginosa. AgNPs showed the highest free radical scavenging activity (79.0 \textpm 0.76%) compared to methanolic leaf extract (68.3 \textpm 0.68%) at 100 g/ml. Further, the cytotoxicity study using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) confirmed that AgNPs successfully inhibited the human colon adenocarcinoma cell line (HT-29) in a dose dependent manner. At higher concentrations (500 g/ml), only 4% of cells survived after 72 hrs of exposure with IC50 value of 120 g/ml. Thus,
these findings offer a new source of biomolecules with diverse biological activities.",
}