Biosynthesis, Characterization and Biological Activities of Silver Nanoparticles from Pogostemon cablin Benth. Methanolic Leaf Extract
This study reports the biosynthesis of silver nanoparticles (AgNPs) using methanolic leaf extract of Pogostemon cablin Benth. (Patchouli) as a reducing agent, and their potent biological (antibacterial, antioxidant and anticancer) activities. The P. cablin extract when
exposed to silver nitrate reduced silver ions to form crystalline AgNPs within 1 h of incubation at room-temperature. UV-visible spectra showed a sharp surface plasmon resonance (SPR) at around 430 nm for the biosynthesized AgNPs and the XRD pattern indicated the crystalline planes of the
face centered cubic silver. The FE-SEM analysis revealed the occurrence of predominant spherical shaped AgNPs with a huge disparity in their particle size distribution with an average size of 25 nm, while, the FTIR data confirmed the bio-reduction and capping of AgNPs by several phytocompounds
present in the methanolic leaf extract. AgNPs effectively inhibited the growth of all the tested human pathogenic bacterial strains (Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli), while, the methanolic leaf extract failed to inhibit the
growth of S. aureus and P. aeruginosa. AgNPs showed the highest free radical scavenging activity (79.0 ± 0.76%) compared to methanolic leaf extract (68.3 ± 0.68%) at 100 μg/ml. Further, the cytotoxicity study using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) confirmed that AgNPs successfully inhibited the human colon adenocarcinoma cell line (HT-29) in a dose dependent manner. At higher concentrations (500 μg/ml), only 4% of cells survived after 72 hrs of exposure with IC50 value of 120 μg/ml. Thus,
these findings offer a new source of biomolecules with diverse biological activities.
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Free Radical Scavenging Activity;
Document Type: Research Article
Department of Surgery, Jinan Municipal Hospital of Traditional Chinese Medicine, Jinan 250012, China
Department of Pharmacy, Binzhou Central Hospital of Shandong, Shandong 251700, China
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
Department of Chemistry, Faculty of Science and Arts, and Promising Center for Sensors and Electronic Devices (PCSED), Najran University, Najran 11001, Kingdom of Saudi Arabia
Department of Botany, Gandhi Faiz-e-Aam College, Shahajahanpur 242001, Uttar Pradesh, India
July 1, 2019
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