The Inhibitory Effects on Cytotoxicity and Nitric Oxide (NO) of the Nano-Encapsulated Extraction of Lipid-Soluble Green Tea Leaves
The purpose of this study was to investigate the bioactive substances and extraction conditions of lipid-soluble green tee extract using supercritical CO extraction from green tea leaves growing in Boseong-gun, Jeollanam-do and to demonstrate cell safety and effects on inflammatory
response of the nano-encapsulated lipid-soluble green tee extract. The polyphenol content of green tea extract using supercritical CO2 extraction was analyzed. The highest polyphenol was extracted in the condition of 400 bar/50 °C. A total of polyphenol content was 2.47 mg/g.
The lipid-soluble vitamin content of supercritical extracts of lipid-soluble green tea was analyzed. Beta-carotene, a vitamin A precursor (4.131 mg/100 g) and vitamin E (537.9 mg/100 g) were detected. ECG content was the highest of catechin of lipid-soluble green tea extract. For cell safety
and NO inhibition, human skin cell-derived HaCaT cells and RBL-2H3 mast cells did not have cytotoxicity in 1% of the nano-encapsulated lipid-soluble green tea leaf extract. RAW 264.7 cells did not show cytotoxicity in 5%. NO production was significantly decreased in RAW 264.7 cells in a concentration-dependent
manner and within the concentration range. Based on the findings, this study suggested the extraction standards and ingredients of polyphenol according to temperature and pressure in extracting green tea leaf ingredients using supercritical CO2 extraction. This study also objectively
demonstrated the safety and anti-inflammatory effect of the nano-encapsulated lipid-soluble green tea leaf extract which is used as functional materials in the cosmetic industry.
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Lipid-Soluble Green Tee Extracts;
Supercritical CO2 Extraction
Document Type: Research Article
Department of Managing, Representative Director B&C Nature Co., Ltd., Seo-gu, Gwangju, 62043, Korea
Department of Food Nutrition, Professor Nambu University, Gwangsan-gu, Gwangju, 62271, Korea
Department of Cosmetic Science, Professor Nambu University, Gwangsan-gu, Gwangju, 62271, Korea
Publication date: February 1, 2019
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Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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