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Optimization of Synthesis and Modification of ZnSe/ZnS Quantum Dots for Fluorescence Detection of Escherichia coil

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This study prepared an innovative 3-mercaptopropionic acid modified ZnSe/ZnS core/shell quantum dots (MPA-ZnSe/ZnS QDs), and established a rapid fluorescence method to detect the E. coli cells count by using MPA-ZnSe/ZnS QDs as fluorescence probe. The formulation variables and process were optimized using the response surface methodology (RSM). Fluorescence microscopy was used to obtain fluorescence microscope images of MPA-ZnSe/ZnS QDs that bind to bacteria. The fluorescence peak intensity increases with increasing cells count in the range of 101–108 CFU/mL. Compared with the traditional based on fluorescent detection methods, this method is more convenient and useful in the bacterial count determination.
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Keywords: Escherichia coli; Fluorescence Measurement; MPA-ZnSe/ZnS Quantum Dots; Response Surface Methodology (RSM)

Document Type: Research Article

Affiliations: 1: Xiang Ya School of Public Health, Central South University, Changsha, Hunan, 410078, China 2: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi, 541014, China 3: Hunan Institute of Food Quality Supervision Inspection and Research, Changsha, Hunan, 410001, China

Publication date: 01 May 2018

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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