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Probing Amyloid β and the Antibody Interaction Using Atomic Force Microscopy

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Amyloid β (Aβ) peptide is considered to be the critical causative factor in the pathogenesis of Alzheimer’s disease (AD) because the hydrophilic molecules accumulated outside of the neural cells and results in the formation of highly toxicity amyloid plaque. In this study, we probed the interaction between Aβ and the antibody using atomic force microscopy (AFM). We compared two kinds of antibodies which are the antibody for Aβ 1-42 (antibody42) and the antibody for Aβ 1-16 (antibody16). To detect the interaction between Aβ and the antibodies, the single molecular force spectroscopy was carried out using Aβ modified glass substrate and the antibodies modified AFM probes. In the results, the single Aβ-antibody42 dissociation constant was estimated to be 5.2 × 10−3 s−1 and the single Aβ-antibody16 dissociation constant was 2.8×10−2 s−1. The Aβ-antibody42 showed 5.3 times longer bond life time compare with Aβ-antibody16. It suggested that antibody42 is better choice for the Aβ sensor development.
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Keywords: Amyloid β Detection; Atomic Force Microscopy; Immunochemistry; Single Molecular Force Spectroscopy

Document Type: Research Article

Affiliations: 1: National Institute for Nanomaterials Technology, Pohang University of Science and Technology, 77, Cheongam-ro, Nam-gu, Pohang, Gyeongbuk, 37673, Korea 2: Top Run R&D Center, Dong Yang Industrial Co. Ltd., 46-14, Okge2gongdan-ro, Gumi, Gyeongbuk, 622-14, Korea 3: Convergence Medical Device Research Center, Gumi Electronics and Information Technology Research Institute, 350-27, Gumidae-ro, Gumi, Gyeongbuk, 39253, Korea

Publication date: February 1, 2018

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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