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Preparation and Characterization of Double-Stranded DNA Brushes via Surface-Initiated Enzymatic Polymerization

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In this study, we have prepared double-stranded DNA brushes on glass or quartz substrates through the immobilization of biotinylated oligo-DNA, followed by hybridization and polymerization of the DNA by a surface-initiated enzymatic polymerization method. The density of the DNA brush was calculated based on UV spectra and agarose gel electrophoresis. Prepared DNA brushes showed a relatively high density of DNA chains (over 1500 chains/μm2) independent of the length of the DNA. Atomic force microscopy of the 2D-patterned DNA brushes indicated dynamic changes in height under various salt concentrations. These results provided insight into the conformation of DNA brushes.
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Keywords: Conformation Change; DNA; Patterning; Polymer Brush; Surface-Initiated Polymerization

Document Type: Research Article

Affiliations: 1: Research Institute for Electronic Science, Hokkaido University, Kita 21, Nishi 10, Kita-Ku, Sapporo 001-0021, Japan 2: Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13, Nishi 8, Kita-Ku, Sapporo 060-8628, Japan

Publication date: December 1, 2017

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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