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A Novel Capturing Method for Quantification of Extra-Cellular Nanovesicles

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Extracellular vesicles (EVs), secreted by cells and found in body fluids play important roles in intercellular communication. Therefore, EVs are receiving increasing attention as potential biomarkers in the diagnosis and prognosis of various diseases. However, the detection and the quantification of EVs are hampered by the nanometer scale of these particles and by the lack of optimized quantification methods. Atomic force microscopy (AFM) is a powerful technology that can detect small particles. Here we report a 3D capture method for sample preparation of AFM which improves the accuracy, sensitivity and reproducibility for EVs’ detection, compared to conventional sample preparation methods. By shaking a mica plate in EV solution, all the EVs were captured onto the 2D surface. The majority of the captured particles have a size ranging from 10 to 120 nm, which correlates with size data obtained from transmission electron microscopy studies. This novel sample preparation method has high adaptability potential and can also be applied to other organic and inorganic nanoparticles.
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Keywords: 3D Capture Method; AFM; Extracellular Vesicle; Quantification; TEM

Document Type: Research Article

Affiliations: 1: Jiangsu Collaborative Innovation Center of Photovoltaic Science and Engineering, School of Petrochemical Engineering, Department of Materials Science and Engineering, Changzhou University, Changzhou 213164, China 2: Department of Pharmacology and Experimental Therapeutics, The Center for Cancer Research, Section of Hematology and Medical Oncology, Boston University School of Medicine, Boston, MA 02118, USA 3: Jiangnan Graphene Research Institute, Changzhou 213149, China 4: C-CINA, Biozentrum Basel, Switzerland; LBR, Paul Scherrer Institute, Villigen, CH-4003, Switzerland

Publication date: February 1, 2017

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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