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Fabrication of Ovalbumin–Phospolipid Thin Film with Minimal Protein Aggregation by Different Self-Assembly Methods

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This paper describes a new approach for the preparation of Ovalbumin (OVA)-phospholipids (DPPC) thin film, where we minimize the aggregation among protein molecules. A comparative study on the films fabricated by self-assembly (SA) of protein-lipid mixed vesicle on hydrophilic glass substrate and SA of protein alone on prefabricated Langmuir-Blodgett (LB) film of DPPC is being carried out. Far UV circular dichroism spectroscopy, FTIR analysis of amide bands and surface morphology study by FE-SEM imaging is being used to examine the extent of conformational change as well as aggregation of protein. Protein-lipid thin film prepared by the former method provides the secondary structure of individual OVA molecules with increment of α-helical segments. In this process, OVA molecules do not show larger aggregation among themselves. However, in the second method, we find unfolded larger aggregated structure of OVA with increment of β-structure of OVA.
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Document Type: Research Article

Publication date: 01 May 2009

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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