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A Review on Some Recent Advances on Membrane Chromatography for Biomolecules Purification

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Membrane chromatography (MC) has been increasingly used these last years and has become a well-established technique of preparative chromatography. The aim of this state of the art review is to present some of the recent developments of this purification technique. First, the main stationary phases used for preparative chromatography are recalled, including resin beds, membranes and monoliths. The second part of the review is devoted to the interaction modes which can be used in MC, such as ion exchange, affinity and hydrophobic interactions. In the next section, advances made on device geometries are summarized including flat sheets, hollow fibers, spiral and crossflow devices. The main advances in MC modelling are then presented with models including dispersive effects in the experimental set-ups and recent computational fluid dynamics (CFD) models. In the last section, several applications of MC are summarized: proteins, monoclonal antibodies and virus purification, as well as other emerging applications like lactoferrin isolation from whey.
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Document Type: Review Article

Publication date: 01 March 2016

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  • Journal of Colloid Science and Biotechnology is an international multidisciplinary peer- reviewed journal covering all aspects of colloid science including colloids preparation, characterization, structure-property relationships, spectroscopy, chemical and physical properties and applications of colloids in biotechnology, medicine and pharmaceuticals. This journal deals with interdisciplinary research areas of chemistry, physics, biology, materials science, polymer science, nanotechnology covering all topics related to colloids such as latexes, emulsions, suspensions, micellar systems, gels, composites, hybrids, surfactants, aerosols, foams, minerals, soft matter, microfluidics, encapsulation processes of active molecules, biomolecules, surfacial and interfacial processes, catalysis, electrochemistry, theoretical aspects, computer simulations and colloids in biotechnology and medicine.
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