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Stabilization of Arthrobacter viscosus Penicillin G Acylase

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A wide array of additives, including polymers, sugars, and amino acids, was examined in order to enhance the Arthrobacter viscosus NRRL B-1973 penicillin G acylase (PGA) thermal stability and also to stabilize PGA against the lyophilization process stresses. Among all the tested additives, diethylaminoethyl dextran (DEAE dextran) was the upmost thermal stabilizer. As it brought about a 14.5 fold increase in the half-life of PGA from 0.931 h (for the control without additives) to 13.535 h and also reduced the first-order thermal denaturation constant at 60 °C from 0.745 h–1 to 0.051 h–1. On the other hand, arginine offered PGA maximum stabilization during the lyophilization process. Consequently, combination of DEAE dextran and arginine into a single formula offered PGA full protection against the lyophilization stresses.
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Keywords: ARGININE; CRYOPROTECTION; DIETHYLAMINOETHYL DEXTRAN (DEAE DEXTRAN); LYOPROTECTION; PENICILLIN G ACYLASE (PGA); POLYMERS; THERMAL STABILITY

Document Type: Research Article

Publication date: December 1, 2013

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  • Journal of Colloid Science and Biotechnology is an international multidisciplinary peer- reviewed journal covering all aspects of colloid science including colloids preparation, characterization, structure-property relationships, spectroscopy, chemical and physical properties and applications of colloids in biotechnology, medicine and pharmaceuticals. This journal deals with interdisciplinary research areas of chemistry, physics, biology, materials science, polymer science, nanotechnology covering all topics related to colloids such as latexes, emulsions, suspensions, micellar systems, gels, composites, hybrids, surfactants, aerosols, foams, minerals, soft matter, microfluidics, encapsulation processes of active molecules, biomolecules, surfacial and interfacial processes, catalysis, electrochemistry, theoretical aspects, computer simulations and colloids in biotechnology and medicine.
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