Provider: Ingenta Connect Database: Ingenta Connect Content: application/x-research-info-systems TY - ABST AU - Mazzaferro, Silvia AU - Bouchemal, Kawthar AU - Maksimenko, Andrey AU - Skanji, Rym AU - Opolon, Paule AU - Ponchel, Gilles TI - Reduced Intestinal Toxicity of Docetaxel Loaded Into Mucoadhesive Nanoparticles, in Mouse Xenograft Model JO - Journal of Colloid Science and Biotechnology PY - 2012-12-01T00:00:00/// VL - 1 IS - 2 SP - 210 EP - 217 KW - MUCOADHESION KW - LDH KW - INTESTINAL TOXICITY KW - PIBCA NANOPARTICLES KW - HISTOLOGICAL ANALYSIS KW - DOCETAXEL KW - USSING CHAMBER N2 - The present study demonstrated that the local intestinal toxicity of docetaxel (Dtx) was low when encapsulated in mucoadhesive nanoparticles composed of methyl-β-cyclodextrin (Me-β-CD)/poly(isobutylcyanoacrylate) coated with thiolated chitosan. The experiments were carried out using intestinal specimens collected from mice with a subcutaneous tumor at an advanced stage in order to obtain challenging pathological conditions. Surprisingly, both Dtx-loaded nanoparticles and unloaded-nanoparticles when administered orally induced a lower level of the intestinal and the colonic inflammations and ulcerations compared to intravenous (i.v) Dtx injection. The small intestine and the colon sections showed a preserved architecture, normal thickness of the mucosa, and normal viability. Furthermore, Dtx-loaded nanoparticles and unloaded-nanoparticles allowed decreasing the release of lactate dehydrogenase (LDH) enzyme of 22% and 43% respectively in comparison with the i.v. Taxotere® administration. These results indicated clearly that: (i) Me-CD/poly(isobutylcyanoacrylate) nanoparticles coated with thiolated chitosan reduced the local intestinal toxicity of Dtx, (ii) unloaded mucoadhesive nanoparticles had a protective effect allowing the preservation of intestinal integrity in mouse xenograft model. UR - https://www.ingentaconnect.com/content/asp/jcsb/2012/00000001/00000002/art00007 M3 - doi:10.1166/jcsb.2012.1022 UR - https://doi.org/10.1166/jcsb.2012.1022 ER -