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Effect of Stimulation by Ultrahigh Molecular Weight Polyethylene Wear Particles on the Osteogenesis Capability of Fibroblasts

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Ultrahigh molecular weight polyethylene (UHMWPE) has been reported to be commonly used in total hip arthroplasty (THA). The aim of our study was to investigate the osteogenesis capacity of synovial membrane-derived fibroblasts cultured with UHMWPE wear particles during THA. Fibroblasts were isolated from femoral neck fracture patients and divided into 2 groups: (1) Fibroblasts and UHMWPE particles co-cultured; (2) Fibroblasts cultured alone as control. Osteogenesis capacity of synovial membrane-derived fibroblasts was investigated using MTT assay, ELISA, western blot and Alizarin Red staining. No significant difference was found in the receptor activator of NFκB (RANK) expression level between the groups (P > 0.05). The levels of alkaline phosphatase (ALP)\osteocalcin (OSC)\Runt-related transcription factor 2 (Runx2)\Osterix expression in the group of fibroblasts cultured with particles were significantly higher (P < 0.05). More calcium accumulated in the group of fibroblasts cultured with particles. Our results indicated that fibroblasts derived from synovial membrane were capable of converting into osteoblast when co-cultured with wear particles from UHMWPE used in THA, which demonstrated that UHMWPE was a useful material for femoral neck fracture patients.
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Keywords: FIBROBLAST; OSTEOGENESIS; OSTEOLYSIS; ULTRAHIGH MOLECULAR WEIGHT POLYETHYLENE; WEAR PARTICLES

Document Type: Research Article

Publication date: 01 May 2018

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  • Journal of Biomaterials and Tissue Engineering (JBT) is an international peer-reviewed journal that covers all aspects of biomaterials, tissue engineering and regenerative medicine. The journal focuses on the broad spectrum of research topics including all types of biomaterials, their properties, bioimplants and medical devices, biofilms, bioimaging, BioMEMS/NEMS, biosensors, fibers, tissue scaffolds, tissue engineering and modeling, artificial organs, tissue interfaces, interactions between biomaterials, blood, cells, tissues, and organs, regenerative medicine and clinical performance.
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