@article {Cai:2018:2157-9083:124,
title = "Fat Mass and Obesity Gene was Potentially Related with Diabetic Retinopathy Through Immune Mechanism",
journal = "Journal of Biomaterials and Tissue Engineering",
parent_itemid = "infobike://asp/jbte",
publishercode ="asp",
year = "2018",
volume = "8",
number = "1",
publication date ="2018-01-01T00:00:00",
pages = "124-131",
itemtype = "ARTICLE",
issn = "2157-9083",
eissn = "2157-9091",
url = "https://www.ingentaconnect.com/content/asp/jbte/2018/00000008/00000001/art00016",
doi = "doi:10.1166/jbt.2018.1723",
keyword = "STZ-TREATED MICE, DIABETIC RETINOPATHY, FTO, TNF-α, FPG",
author = "Cai, Minyun and Tan, Haibo and Gu, Liqiong and Cui, Hongping",
abstract = "The role of inflammation in the development and progression of diabetic retinopathy (DR) has been studied for a long time, but in the last 10 years there has been enormous interest in the molecular mechanisms to develop novel therapeutic approaches. Of this study, there were none of
significantly differences of Age, Sex, Body mass index, TC, TG and CRP in different groups. However, FPG values and HbA1c values in patients with/without DR were significantly higher than that in healthy group. Meanwhile, TNF- contents in patients with DR were also significantly
higher than that in patients without DR group. Over-expressed FTO gene in HepG cell resulted in a significant increase in mRNA and protein expression of TNF- compared with that in wild group. Knockdown FTO gene in HepG cell had significantly reduce TNF- expression
compared with Control group, which indicated that knock-down FTO gene in HepG cell had significantly effects on TNF- expression. Of the RNA dot-blot analysis, over-expressed FTO gene in HepG cell significantly promoted m6A expression. Meanwhile, knockdown FTO gene
in HepG cell had significantly reduce m6A expression compared with Control group. The results revealed that knockdown and over-expressed FTO gene in HepG cell could effectively affect the m6A expression, which indicated the positive relationships between FTO gene and
m6A in vitro. In addition, Retinal vessel in STZ-induced mice resulted in a significantly increase in mRNA and protein expression of TNF- compared with that in wild group, which indicated that STZ-treated mice significantly promoted TNF- expression.
The results mentioned above revealed that STZ-induced treatment could effectively affect the m6A and TNF- expression, which indicated the positive relationships between FTO gene and m6A in vivo. Characterization of the role of FTO gene in the development
of DR should lead to a better understanding.",
}