Engineering Bacterial Cellulose with Bone Marrow Stem Cells and Meniscal Fibrochondrocytes for Meniscal Repair
Meniscal tears are the most common knee injuries caused by a rupture of one or more of the fibrocartilage strips in the knee, which causes pain in the joint. Partial or complete meniscal replacement with engineered materials has gained increasing attention. This study tries to characterize a bacterial cellulose (BC) for potential use in meniscal repair. The toxicity of BC was assayed by MTT after cells were cultured with BC extraction medium. Cell growth on BC sheets and in vitro differentiation of bone marrow stem cells (BMSCs) into meniscal fibrochondrocytes (MFCs) were analyzed by HE staining, quantitative RT-PCR, ICC, and scanning electron microscopy. The serum IL-6 and TNF-α levels were measured by ELISA, macrophage infiltration by CD68 IHC, and tissue repair by HE staining. When co-cultured with MFCs, BMSCs were induced to differentiate into MFCs. The extraction medium of BC sheets did not inhibit MFC growth. Implantation of MFC and BMSC-engineered BC sheets into rabbit buttock muscles did not elicit inflammatory reactions and supported injury recovery. The bacterial cellulose exhibited great potential to be engineered for meniscal repair due to its biocompatability, non-immunoactivity, and bioactivity to support BMSC and MFC growth and differentiation.
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Document Type: Research Article
Publication date: February 1, 2015
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- Journal of Biomaterials and Tissue Engineering (JBT) is an international peer-reviewed journal that covers all aspects of biomaterials, tissue engineering and regenerative medicine. The journal focuses on the broad spectrum of research topics including all types of biomaterials, their properties, bioimplants and medical devices, biofilms, bioimaging, BioMEMS/NEMS, biosensors, fibers, tissue scaffolds, tissue engineering and modeling, artificial organs, tissue interfaces, interactions between biomaterials, blood, cells, tissues, and organs, regenerative medicine and clinical performance.
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