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Fluid Shear Stress Induces Differential Gene Expression of Leukemia Inhibitory Factor in Human Mesenchymal Stem Cells

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The multi-potentiality of human mesenchymal stem cells (MSCs) makes them ideal candidates for tissue engineering of musculoskeletal tissues. Mechanical forces like fluid shear stress are important regulators of cell behaviour and activity in such tissues, although how they influence MSCs is not fully understood. Herein, we investigate in human MSCs the effect of fluid shear stress on cytokine gene expression. MSCs cultured in vitro were exposed to different profiles of fluid shear stress using a bioreactor. Cytokine gene expression was analysed with DNA microarrays and quantitative real-time RT-PCR. Data were analysed using pathway analysis and repeated measures analysis of variance. Fluid shear stress induced differential expression of a total of 46 different cytokine genes. In particular, we observed consistent and substantial up-regulation of leukemia inhibitory factor (LIF; 2–90-fold) and interleukin-11 (IL-11; 3–56-fold), both IL-6 class cytokines, and also of IL-1β (2–150-fold). Preliminary microarray data indicating up-regulation of several chemokines could not be verified. Our findings indicate fluid shear stress is a potent inducer of cytokine gene expression in human MSCs. LIF/IL-6 class cytokine genes in particular are sensitive to shear stress, suggesting they are important in the response elicited by MSCs.
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Keywords: CHEMOKINES; CYTOKINES; FLUID SHEAR STRESS; GENE EXPRESSION; LEUKEMIA INHIBITORY FACTOR; MESENCHYMAL STEM CELLS

Document Type: Research Article

Publication date: December 1, 2011

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  • Journal of Biomaterials and Tissue Engineering (JBT) is an international peer-reviewed journal that covers all aspects of biomaterials, tissue engineering and regenerative medicine. The journal focuses on the broad spectrum of research topics including all types of biomaterials, their properties, bioimplants and medical devices, biofilms, bioimaging, BioMEMS/NEMS, biosensors, fibers, tissue scaffolds, tissue engineering and modeling, artificial organs, tissue interfaces, interactions between biomaterials, blood, cells, tissues, and organs, regenerative medicine and clinical performance.
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