Near Infrared Visible Biocompatible Particles Maintain Optical Signal in Dystrophic Muscle
Background: Near Infrared (NIR) active biocompatible particles have great potential to aid in the understanding of the delivery of therapeutic agents in the muscular dystrophies. Currently, tracking of such particles remains primarily performed through ex vivo analysis. The combination of clinically used compounds, such as indocyanine green (ICG) and poly-lactic acid (PLA), to compose NIR active particles that can be tracked in vivo could serve this need. Methods: ICG was encapsulated in PLA based particles and optical stability was assessed both in aqueous solution and in vivo. ICG-PLA particles were delivered subcutaneously and intramuscularly into the hindlimbs of control and dystrophic mice. Results: ICG-PLA particles (205±78 nm) were produced and demonstrated fluorescence properties similar to aqueous ICG. ICG-PLA particles had a half-life up to 82 fold greater than that of aqueous ICG in vitro. Similarly, ICG loaded particles demonstrated prolonged fluorescence for up to four weeks following intramuscular injections into dystrophic mice, whereas aqueous ICG fluorescence was extinguished 15 times quicker, returning to baseline fluorescence levels within one week. Conclusion: ICG-PLA biocompatible particles have increased stability and permit prolonged optical imaging in dystrophic muscle. This NIR platform has the potential to track particle distribution in vivo in a reliable, longitudinal safe, inexpensive, non-invasive, and facile manner.
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Document Type: Research Article
Publication date: 01 May 2017
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