@article {Park:2013:1550-7033:880,
title = "Microfluidic Chip for the Detection of Biological Toxic Effects of Polychlorinated Biphenyls on Neuronal Cells",
journal = "Journal of Biomedical Nanotechnology",
parent_itemid = "infobike://asp/jbn",
publishercode ="asp",
year = "2013",
volume = "9",
number = "5",
publication date ="2013-05-01T00:00:00",
pages = "880-885",
itemtype = "ARTICLE",
issn = "1550-7033",
url = "https://www.ingentaconnect.com/content/asp/jbn/2013/00000009/00000005/art00016",
doi = "doi:10.1166/jbn.2013.1497",
keyword = "MICROFLUIDIC DEVICE, PERSISTENT ORGANIC POLLUTANTS (POPS), PC12, NEURAL CELL CHIP, POLYCHLORINATED BIPHENYLS (PCBS)",
author = "Park, Soeun and Choi, Jeong-Woo and Kim, Young-Kee",
abstract = "The aim of this study was to develop a microfluidic neuronal cell chip device to monitor the toxic effects of polychlorinated biphenyls (PCBs) on PC-12 neuronal cells. PCBs adversely influence the activities of neuronal cells in the nervous system. In PC-12 cells, the production and
secretion of dopamine decreases in response to PCB exposure. The microfluidic device that we developed to measure the amount of dopamine by cyclic voltammetry is composed of a control layer, a fluidic layer, and a gold electrode-patterned glass wafer. The control channel in the control layer
functions as a microvalve to control the flow of the fluidic channel in the fluidic layer. The fluidic layer consists of 3 reaction chambers as well as fluidic channels. Three electrodes, including the working electrode, counter electrodes, and a reference electrode, are placed in a fluidic
chamber. The electrochemical signals of dopamine, either from a standard dopamine solution or from the culture supernatant from cultured PC-12 cells, were obtained using a fabricated microfluidic neuronal cell chip by cyclic voltammetry. When PCBs were added to cultures of PC-12 cells, the
amount of dopamine secreted from the PC-12 cells decreased due to the reduced activity of PC-12 cells. The fabricated neuronal cell chip was capable of detecting the toxic effect of dopamine on neuronal cells at concentrations of 10 g/L and over. The practicality of the developed
microfluidic neuronal cell chip was validated using river water spiked with PCBs.",
}