Skip to main content
padlock icon - secure page this page is secure

Sensing Interactions Between Vimentin Antibodies and Antigens for Early Cancer Detection

Buy Article:

$105.00 + tax (Refund Policy)

This paper examines the use of polysilicon microcantilevers in the bioassay of Vimentin antigens that are presented in human-osteosarcoma (HOS) cells. Pre-calibrated atomic force microscopy (AFM) microcantilevers were functionalized with Vimentin antibodies using a two-step process that involved the application of an amine-rich polyethyleneimine (PEI) to the silicon oxide layer that is presented on unpassivated silicon surfaces. This is followed by a second step reaction between the amines at the surface and the carboxylic acid groups in the amino acid unit of the Vimentin antibody. The possible interaction between Vimentin antibody on microcantilevers and Vimentin antigens were then studied in a biofluidic chamber attached to an AFM platform. These were explored by exposing the Vimentin antibodies to increasingly small concentrations of Vimentin antigens. Static deflection in the microcantilevers was measured as a result of the surface stresses that are induced by changes in the molecular structure due to antigen-antibody interactions. The implications of the results were discussed for the early detection of cancer in biopsies.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: AFM; ANTIBODY; ANTIGEN; ANTIGEN-ANTIBODY INTERACTIONS; BIOMOLECULE; MICROCANTILEVER; SURFACE STRESSES; VIMENTIN

Document Type: Research Article

Publication date: 01 March 2005

More about this publication?
  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more