Fermentation Engineering for Enhanced Paclitaxel Production by Taxus Media Endophytic Fungus MF-5 ( Alternaria sp.)
Taxol is the most widely used antitumor agent that was industrialized in the past decades. It has been globally used for efficient cancer treatment. A 32 fungus was isolated from five-year-old female Taxus yew and male Mantilla yew trees and screened for Taxol production. Only
Alternaria alternata MF5 was shown to produce Taxol, as isolated from the bark of female Taxus yew. The fungus was recognized based on the morphological characters, DNA sequencing and Taxol production screening through HPLC. The MZ medium supplemented with 10 mg/L CuSO4,
20 mg/L MeJA and 5 mg/L GA was most effective for high yield of Taxol in the fermenter. The quantity of Taxol by Alternaria alternata MF5 was 5.7 mg/L after 60 h, as quantified by HPLC and found to be 11-fold higher when compared with other findings. Therefore, the isolated fungus can
assist as a possible biomass for fungus business, to improve Taxol market production.
Keywords: ALTERNARIA ALTERNATA; FERMENTER; PRODUCTION; TAXOL; TAXUS YEW
Document Type: Research Article
Publication date: 01 December 2018
- The goal of the creation of a biobased economy is challenging to agriculture, forestry, academia, government and industry. The extractable resources of the Earth are finite, regardless of the quibble over when they will be depleted. The economic, political and social demands for biobased chemicals, materials and energy are expected to radically transform the materials industries, particularly the plastics industry as well as the biofuel industry. These changes will be based on the principles of sustainability, eco-efficiency, industrial ecology, and green chemistry and engineering. In keeping with the growth of knowledge in this field, there is a strong need for a forum to share original research related to biobased materials and bioenergy. The Journal of Biobased Materials and Bioenergy (JBMB) has been created as an international peer-reviewed periodical to fulfill the need for communication in these research areas. This journal will encompass related research activities in all fields of science, engineering and the life sciences.
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