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Effects of C. ramiflora Linn. Leaf Extract Against Dengue Virus Replication In Vitro on Huh7it-1 Cell

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Introduction: Dengue virus (DENV) causes an acute infection that may lead to death. Due to the morbidity and mortality produced, dengue infection is still a serious health problem especially in tropical countries. To this time, there is still no specific antiviral to overcome DENV infection. Therefore, a research to find a specific antiviral for DENV is necessary. This research is aimed to evaluate the effect of Cynometra ramiflora Linn. leaf extract In Vitro on Huh7it-1 cell as a specific antiviral for DENV infection. Methods: An experimental research was conducted in August 2014–June 2015. The leaf extract of C. ramiflora Linn. was diluted to concentration 40 μg/ml, 20 μg/ml, 10 μg/ml, 5 μg/ml, 2,5 μg/ml, and 1,25 μg/ml. Next, DENV was exposed to each concentration. The inhibition of DENV replication (IC50) was observed using Focus Assay, while the toxicity of the extract to Huh7it-1 (CC50) was evaluated using MTT Assay. Data were analyzed using T -test and One Way ANOVA. Results: From the experiment, the value of CC50 and IC50 are 125 μg/ml and 20,1 μg/ml, respectively. Conclusion: From the research, it is concluded that C. ramiflora Linn. extract is not toxic and has a potency as antiviral for DENV.

Keywords: CC50; Cynometra Ramiflora Linn; DENV; IC50

Document Type: Research Article

Affiliations: 1: Faculty of Medicine, Universitas Indonesia, Indonesia, Central Jakarta, Indonesia 2: Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Indonesia, Central Jakarta, Indonesia

Publication date: July 1, 2017

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