Toxicity and Transfection Efficiency of New Non-Viral Delivery Systems for Small Non-Coding RNAs: Amphiphilic Poly(N-Vinylpyrrolidone) and Diethylaminoethyl-Dextran-Methacrylic Acid Methyl Ester Copolymer
Treatment with small regulatory nucleotides is a new strategy in modern medicine. However, administering naked nucleotides is challenging due to their rapid degradation by RNases within and outside of cells. Recently, many delivery systems for the transporting of nucleotides have been synthesized. Most of these systems have limitations. In some cases, these systems may be costly to produce; in other cases, they may be toxic, cause septicaemia or exhibit ineffective complex formation, rapid biodegradation, or a low index of intracellular entry. Thus, the need to synthesize of new effective, protective carriers for nucleotides is increasing. In this study, some of the properties of two polymeric systems for delivering the small non-coding RNAs miR-155 were compared. Cells were treated with amphiphilic poly(N-vinylpyrrolidone) (PNVP) and diethylaminoethyl-dextran-methacrylic acid methyl ester copolymer (DDMC), and their toxicities and transfection dynamics were compared using the MTT assay and analysis of the expression of the fluorescent protein mKate2, respectively. The optimal doses and ratios of nucleotides and polymeric carriers were chosen. The studied polymers effectively transfected cancer cells.
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Document Type: Research Article
Publication date: 01 May 2017
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