Skip to main content

Free Content Maximum Entropy Method for Analyses of Fluorescence Correlation Spectra of Oligonucleotide Intra-Chain Collision

It has been a challenge to accurately extract dynamic information from experimental fluorescence correlation spectroscopy (FCS) data. In this paper, we compare three major fitting methods: the model-dependent multiple exponential function (MultiExp), the empirical stretched exponential function (StreExp), and the exponential function based on the model-free maximumentropy method (MemExp). MultiExp has straight forward physical significance but it is difficult to implement and interpret in a complex system. StreExp has simple form and is easy to use but its physical picture is obscure. MultiExp is model free but its results are sensitive to experimental noise. A good choice seems to be a combination of MemExp and MultiExp. In our example, we have unraveled that two independent processes exist in the intra-chain collision of a single-stranded DNA when base pair formation is possible, which has not been observed by previous investigators. MemExp is recommended for the FCS data analysis, although caution should be exercised in the practice.

Keywords: Fluorescence correlation spectroscopy; Maximumentropy method; Oligonucleotide; Photoinduced electron transfer; Relaxation time distribution; Stretched exponential function

Document Type: Research Article

Publication date: April 15, 2010

More about this publication?
  • Acta Physico-Chimica Sinica, founded in 1985, is sponsored by the Chinese Chemical Society and organized by the College of Chemistry and Molecular Engineering, PekingUniversity. Since 1997, Acta Physico-Chimica Sinica has been indexed in SCI of ISI (US). Acta Physico-Chimica Sinica is devoted to reporting new and original experimental and theoretical basic research of interest to physical chemists, biophysical chemists, and material physical chemists. Manuscripts that are essentially reporting data, applications of data, or reviews of the literature are not suitable for publication in Acta Physico-Chimica Sinica.
  • Editorial Board
  • Information for Authors
  • Submit a Paper
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content