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Open Access Novel Two-Stage Fine Milling Enables High-Throughput Determination of Glyphosate Residues in Raw Agricultural Commodities

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Dramatic process-efficiency gains for residue analysis of glyphosate in raw agricultural commodities (RACs) were achieved by development and validation of a two-stage fine-milling process. This secondary milling produced a uniform and consistent product that could be reproducibly measured with 75 mg analytical test portions. The milligram scale sample size enabled the direct weighing of sample into a liquid-handler-compatible 96-well format. A high-throughput workflow based on this innovative comminution approach for the quantitation of glyphosate, a nonselective herbicide, and its main degradation product, aminomethylphosphonic acid, was validated in various RACs and used to demonstrate the applicability of the two-step milling process. The precision and reproducibility of 75 mg analytical portions taken through this assay was used to demonstrate the feasibility of using a two-stage fine-milling technique for pesticide residue applications. An RSD of less than 10% was achieved in endogenous glyphosate residues in multiple RACs. Comparable recoveries and superior precision were achieved with this new method as compared with a validated 10 g scale method.
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Document Type: Research Article

Affiliations: Monsanto Company, 700 Chesterfield Pkwy West, Chesterfield, MO 63017, USA

Publication date: 01 May 2018

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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