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Validated HPLC-Diode Array Detector Method for Simultaneous Evaluation of Six Quality Markers in Coffee

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Nicotinic acid, N-methylpyridinium ion, and trigonelline are well studied nutritional biomarkers present in coffee, and they are indicators of thermal decomposition during roasting. However, no method is yet available for their simultaneous determination. This paper describes a rapid and validated HPLC-diode array detector method for the simultaneous quantitation of caffeine, trigonelline, nicotinic acid, N-methylpyridinium ion, 5-caffeoylquinic acid, and 5-hydroxymethyl furfural that is applicable to three coffee matrixes: green, roasted, and instant. Baseline separation among all compounds was achieved in 30 min using a phenyl-hexyl RP column (250 × 4.6 mm, 5 μm particle size), 0.3% aqueous formic buffer (pH 2.4)–methanol mobile phase at a flow rate of 1 mL/min, and a column temperature at 30°C. The method showed good linear correlation (r2 > 0.9985), precision (less than 3.9%), sensitivity (LOD = 0.023–0.237 μg/mL; LOQ = 0.069–0.711 μg/mL), and recovery (84–102%) for all compounds. This simplified method is amenable for a more complete routine evaluation of coffee in industry.
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Document Type: Research Article

Affiliations: Pontificia Universidad Catolica del Peru (PUCP), Departamento de Ciencias–Quimica, Av. Universitaria 1801, Lima 32, Peru

Publication date: January 1, 2015

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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