A Rapid Method for Quantifying Chlorogenic Acid Levels in Potato Samples
Chlorogenic acids (CGA) are found in many plant-derived foodstuffs and are claimed to have various beneficial effects on human health. Potatoes are a major component of the human diet and contain CGA, but little is known about their abundance in these important tubers. We therefore used a rapid, sensitive, and selective method for quantifying CGAs in food using ultra-high performance LC diode array detection (UHPLC-DAD). We also established an optimized sample preparation protocol based on ultrafiltration and used these techniques to quantify the CGA contents of selected potato varieties (fresh and after storage) and potato products. The measured CGA concentrations in potato skins were 37–636 mg/100 g dry weight (DW) and were three to four times greater than those in the flesh. Storage reduced the CGA levels in potatoes by up to 81%. The studied potato purees contained 4–11 mg CGA/100 g DW. In addition, the quinic acid contents of potato flesh (11–95 mg/100 g DW) and puree (11–22 mg/100 g DW) were measured using stable isotope dilution analysis. None of the tested samples contained caffeic acid. Overall, our results demonstrated that the UHPLC-DAD method can be used to rapidly quantify CGA levels in potatoes and related food products.
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Document Type: Research Article
Affiliations: University of Kaiserslautern, Department of Chemistry, Division of Food Chemistry and Toxicology, Erwin-Schroedinger-Str. 52, 67663 Kaiserslautern, Germany
Publication date: May 1, 2014
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- The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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