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Determination of Organophosphorus and Carbamate Insecticides in Fresh Fruits and Vegetables by High-Performance Thin-Layer Chromatography-Multienzyme Inhibition Assay

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HPTLC-enzyme inhibition assay was applied to different fruit and vegetable samples after individual spiking with organophosphate and carbamate pesticides at their maximum residue limits documented by the European Commission. Samples were extracted according to the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method, including cleanup by primary secondary amine sorbent. Additional cleanup was performed on the HPTLC plate by a prechromatographic step to separate most coextracted matrix compounds from 20 different pesticides under study. With both rabbit liver esterase and cutinase from Fusarium solani pisi as enzyme sources, mean recoveries from apples, cucumbers, grapes, nectarines, plums, tomatoes, and lemons were in the ranges 86–109, 95–129, 96–114, and 90–111% for chlorpyrifos, paraoxon, parathion, and pirimicarb, respectively, with a mean RSD of 8.5% for all samples.
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Document Type: Research Article

Publication date: 01 September 2012

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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