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Quantitative 1H-NMR Spectrometry Method for Quality Control of Aloe vera Products

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An 1H-NMR spectrometry method was developed and validated to quantify acetylated polysaccharides, glucose, malic acid, lactic acid, and acetic acid in Aloe vera products. Nicotinamide was selected as an internal standard (IS) in this quantification method. The quantity of marker compounds was calculated by the relative ratio of the integrals of each compound to the IS. The method validation included specificity, linearity, accuracy, robustness, reproducibility, LOD, and LOQ on two samplesA. vera inner leaf standard and decolorized aloe whole leaf standard from the International Aloe Science Council. Eighteen A. vera commercial products were analyzed with this NMR spectrometry method.
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Document Type: Research Article

Affiliations: 1: Unigen, Inc., 2660 Willamette Dr NE, Lacey, WA 98516. 2: Spectral Service, Laboratorium fr Auftragsanalytik GmbH, Emil-Hoffmann-Strae 33, D-50996 Kln, Germany. 3: Saint Martin's University, Department of Chemistry, 5300 Pacific Ave, Lacey, WA 98503.

Publication date: 01 May 2010

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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