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Evaluation of Chlorhexidine on the Quality of the Hybrid Layer in Noncarious Primary Teeth: An In Vitro Study

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It has recently been observed that chlorhexidine has the capacity to inhibit matrix metalloproteinase. Therefore, the object of this study was to assess the effect of chlorhexidine on the quality of the hybrid layer of noncarious primary teeth. In group 1, the teeth were subjected to acid-etching, chlorhexidine application, Single Bond adhesive insertion, and restoration with resin composite Z250. Group 2 received the same procedures, without the application of the antimicrobial agent. Twenty-five regions were examined by scanning electronic microscopy by blind examiners. The data obtained were statistically analyzed by the chi-square and student t tests at a 5% level of significance. The groups presented few interfacial gaps without statistically significant differences. Group 1 presented a larger number of areas with a visible hybrid layer (68%) vs group 2 (52%). The layer's thickness was 3.33 m and 3.28 m for groups 1 and 2, respectively (P=.94). The results showed that the clinical restorative protocol with the use of chlorhexidine application does not interfere significantly in the morphological characteristics of the hybrid layer.
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Keywords: CHLORHEXIDINE; HYBRID LAYER; MATRIX METALLOPROTEINASES

Document Type: Research Article

Affiliations: 1: Department of Pediatric Dentistry, School of Dentistry, Catholic University of Brasilia, Brasilia, Brazil 2: Department of Pediatric Dentistry, School of Dentistry, University of Brasilia, Brasilia, Brazil 3: Professor of graduate program of University of Brasilia, Brasilia, Brazil

Publication date: January 1, 2010

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  • Acquired after the merger between the American Society of Dentistry for Children and the American Academy of Pediatric Dentistry in 2002, the Journal of Dentistry for Children (JDC) is an internationally renowned journal whose publishing dates back to 1934. Published three times a year, JDC promotes the practice, education and research specifically related to the specialty of pediatric dentistry. It covers a wide range of topics related to the clinical care of children, from clinical techniques of daily importance to the practitioner, to studies on child behavior and growth and development. JDC also provides information on the physical, psychological and emotional conditions of children as they relate to and affect their dental health.
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