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Open Access Cryopreservation of Mauritian Cynomolgus Macaque (Macaca fascicularis) Sperm in Chemically Defined Medium

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The objective of this study was to optimize cryopreservation of sperm from Mauritian cynomolgus macaques (MCM) in defined conditions. Sperm viability and motility were compared between sperm cryopreserved in chemically-defined freezing media with variable osmolarity and the presence of either ethylene glycol or glycerol. The highest percentage viability (after freeze-thaw) was seen in sperm samples that were cryopreserved in medium with an osmolarity of 310 mOsm, while higher osmolarities markedly decreased sperm viability. Ethylene glycol and glycerol at concentrations of 4.6% and 5%, respectively, preserved sperm viability to an equivalent degree. Although higher motility rates and higher straight-line velocities were observed in sperm samples frozen in glycerol compared with ethylene glycol, these differences were not statistically significant. Thawed sperm frozen in defined conditions with glycerol were capable of fertilizing MCM oocytes in vitro, with development to the blastocyst stage. The protocol described here provides an effective method for cryopreservation of sperm to facilitate subsequent in vitro fertilization and genome editing of embryos in MCM species.
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Document Type: Research Article

Affiliations: 1: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 2: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin; Department of Comparative Biosciences, University of Wisconsin-Madison, Madison, Wisconsin; Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, Wisconsin 3: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin; Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin;, Email: [email protected]

Publication date: November 1, 2020

This article was made available online on September 2, 2020 as a Fast Track article with title: "Cryopreservation of Mauritian Cynomolgus Macaque (Macaca fascicularis) Sperm in Chemically Defined Medium".

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  • The Journal of the American Association for Laboratory Animal Science (JAALAS) serves as an official communication vehicle for the American Association for Laboratory Animal Science (AALAS). The journal includes a section of refereed articles and a section of AALAS association news. The mission of the refereed section of the journal is to disseminate high-quality, peer-reviewed information on animal biology, technology, facility operations, management, and compliance as relevant to the AALAS membership. JAALAS accepts research reports (data-based) or scholarly reports (literature-based), with the caveat that all articles, including solicited manuscripts, must include appropriate references and must undergo peer review.

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