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Open Access Underwater Anesthesia of Diamondback Terrapins (Malaclemys terrapin) for Measurement of Auditory Evoked Potentials

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Investigations into the biology of aquatic and semiaquatic species, including those involving sensory specialization, often require creative solutions to novel questions. We developed a technique for safely anesthetizing a semiaquatic chelonian species, the diamondback terrapin (Malaclemys terrapin), for measurement of auditory evoked potentials while animals were completely submerged in water. Custom-modified endotracheal tubes were used to obtain a watertight seal on both sides of the glottis and prevent aspiration of water during testing. No adverse effects were seen after the procedures, and assessment of venous blood-gas partial pressures and lactate concentrations indicated that sufficient gas exchange was maintained under anesthesia through manual ventilation.

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Document Type: Research Article

Affiliations: 1: North Carolina State University, Center for Marine Sciences and Technology, Morehead City, North Carolina, USA 2: Duke University Marine Laboratory, Nicholas School of the Environment, Beaufort, North Carolina, USA 3: Drexel University, Philadelphia, Pennsylvania, USA 4: North Carolina State University, Center for Marine Sciences and Technology, Morehead City, North Carolina, USA. [email protected]

Publication date: November 1, 2013

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  • The Journal of the American Association for Laboratory Animal Science (JAALAS) serves as an official communication vehicle for the American Association for Laboratory Animal Science (AALAS). The journal includes a section of refereed articles and a section of AALAS association news. The mission of the refereed section of the journal is to disseminate high-quality, peer-reviewed information on animal biology, technology, facility operations, management, and compliance as relevant to the AALAS membership. JAALAS accepts research reports (data-based) or scholarly reports (literature-based), with the caveat that all articles, including solicited manuscripts, must include appropriate references and must undergo peer review.

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