Activities of Bio-Larvacides from Symbiont Bacterial of Soft Coral Sarcophyton sp. SCRTG4P4 Against Aedes aegypti

Background: Synthetic insecticides are known as the substances that can lead to an destabilization of ecosystem and enhance a resistance to insecticides. Bio larvacides is an eco-friendly insecticide that can be an alternative vector control for Aedes aegypti. The objective of the research was to determine the activity of biolarvacides of symbiont bacterial soft coral Sarcophyton sp. SCRTG4P4 against Ae. aegypti and molecular identification of Symbiont bacterial of soft coral Sarcophyton sp. Method: This research was done by an experimental method with post-test only control group design. The variation concentrations of symbiont bacterial extract, soft coral SCRTG4P4 (0.15; 0.20; 0.25; 0.30; 0.35; 0.40; 0.45%) and control, were applied to the III–IV instar of Ae. aegypti larva. Data analysis carried out with one-way ANOVA followed by Probit Analysis. Moleculer identification used PCR amplification and DNA sequencing. Results: The result showed that there were significant differences in various concentration of extract soft coral Sarcophyton sp. SCRTG4P4 (p = 0.000) and LC₅₀ values of 0.275% and LC₉₀ values of 0.40%. Based on the result of ANOVA analysis, there were significant differences of various concentration extract as biolarvacides with significance p = 0.000. Molecular identification showed that SCRTG4P4 was similar with Bacillus subtilis. Conclusion: SCRTG4P4 as symbiont bacteria of soft coral Sarcophyton sp. had activities of biolarvacide and it was similar to Bacillus subtilis.