Cryopreservation of in Vitro-grown Shoot Tips of Chinese Medicinal Plant Atractylodes macrocephala Koidz. Using a Droplet-vitrification Method
OBJECTIVE: The aim of this research was to develop an efficient droplet-vitrification protocol for A. macrocephala shoot tips which could be used as a strategy for long-term conservation within gene banks.
MATERIALS AND METHODS: The duration of preculture, loading, and PVS2 steps, as well as the recovery medium formulation, were optimized to achieve high levels of survival and regrowth for A. macrocephala shoot tips after liquid nitrogen exposure.
RESULTS: Survival and regrowth levels after cryopreservation in the cultivar 'Baizhu' were as high as 76% and 62%, respectively. Thermal analysis using differential scanning calorimetry suggested that the PVS2 treatment plays a critical role for successful cryopreservation.
CONCLUSION: The droplet-vitrification method established in this study could be used to cryopreserve A. macrocephala.
Keywords: ATRACTYLODES MACROCEPHALA KOIDZ; DROPLET-VITRIFICATION; REGROWTH; SHOOT TIPS; SURVIVAL; THERMAL ANALYSIS
Document Type: Research Article
Publication date: 01 May 2015
CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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